Differential Mobility Separation for Improving Lipidomic Analysis by Mass Spectrometry SelexION Technology on QTRAP Systems
نویسندگان
چکیده
In recent years, the biochemical study of lipids has transformed from slow multi-dimensional chromatographic separations and chemical derivatization strategies to higher throughput analysis by mass spectrometry. Advances in mass spectrometry have enabled in-depth lipidomic analyses with unparalleled qualitative and quantitative sensitivity. However, unambiguous identification and quantitation of lipid molecular species in total lipid extracts has proven to be difficult, primarily due to isobaric overlapping isobaric and isomeric species. There are greater than 100,000 lipid molecular species present in a typical biological lipid extract that occupy a narrow mass range (~400-1100 amu), making such overlap a significant problem.
منابع مشابه
Quantitation of Limaprost, an Analogue of PGE1 in Human Plasma A highly simplified and sensitive assay using the QTRAP® 6500+ LC-MS/MS system and the SelexION®+ differential mobility separation technology device
متن کامل
Using Differential Mobility Spectrometry to Separate and Localize Sites of Post-Translational Modifications on Peptides
Characterization of the post-translational modification sites (PTMs) on proteins and peptides is a challenging task. Mass spectrometry can provide a good solution for characterization because of the high quality MS/MS spectra that can be obtained for identification and site localization. However, isomeric peptides with PTMs on different residues of the same peptide sequence can be hard to disti...
متن کاملSeparation of Diastereomeric Flubatine Metabolites using SelexION Technology
Key Challenges of Metabolite Separation 1. Metabolite analysis features complex matrices. Samples from plasma and urine are common, but also from in vitro metabolite studies using liver microsomes, possibly causing elevated background signals or isobaric interferences. 2. Enzymatic metabolization often results in a set of isobaric compounds. MS/MS fragmentation can differentiate constitutional ...
متن کاملDetection of Radiation-Exposure Biomarkers by Differential Mobility Prefiltered Mass Spectrometry (DMS-MS).
Technology to enable rapid screening for radiation exposure has been identified as an important need, and, as a part of a NIH / NIAD effort in this direction, metabolomic biomarkers for radiation exposure have been identified in a recent series of papers. To reduce the time necessary to detect and measure these biomarkers, differential mobility spectrometry - mass spectrometry (DMS-MS) systems ...
متن کاملHigh-Throughput and Comprehensive Lipidomic Analysis Using Ultrahigh-Performance Supercritical Fluid Chromatography-Mass Spectrometry.
New analytical approach for high-throughput and comprehensive lipidomic analysis of biological samples using ultrahigh-performance supercritical fluid chromatography (UHPSFC) with electrospray ionization-mass spectrometry (ESI-MS) is presented in this work as an alternative approach to established shotgun MS or high-performance liquid chromatography-MS. The lipid class separation is performed b...
متن کامل